3EUH

Crystal Structure of the MukE-MukF Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-12
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	77.338, 151.146, 188.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.225
R-free	0.27400
Structure solution method	SIRAS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.065	0.048	0.227
Number of reflections	59926
<I/σ(I)>	31.821
Completeness [%]	97.1	98.3	92.6
Redundancy	6.1	7.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	287	PEG 400, CaCl2, MgAcet, glycine, pH 6.5, vapor diffusion, temperature 287K
1	VAPOR DIFFUSION	6.5	287	PEG 400, CaCl2, MgAcet, glycine, pH 6.5, vapor diffusion, temperature 287K
1	VAPOR DIFFUSION	6.5	287	PEG 400, CaCl2, MgAcet, glycine, pH 6.5, vapor diffusion, temperature 287K