3EUC
Crystal structure of histidinol-phosphate aminotransferase (YP_297314.1) from RALSTONIA EUTROPHA JMP134 at 2.05 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.579, 93.433, 111.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.988 - 2.050 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.671 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.988 | 29.990 | 2.100 |
High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
Rmerge | 0.102 | 0.036 | 0.758 |
Total number of observations | 1966 | 13492 | |
Number of reflections | 50845 | ||
<I/σ(I)> | 8.8 | 27.4 | 1.6 |
Completeness [%] | 100.0 | 96.7 | 100 |
Redundancy | 3.6 | 3.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 0.8M (NH4)2SO4, 0.1M Citrate pH 4.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |