3EUB
Crystal Structure of Desulfo-Xanthine Oxidase with Xanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 73.298, 133.176, 142.633 |
Unit cell angles | 96.88, 93.11, 90.02 |
Refinement procedure
Resolution | 33.080 - 2.600 |
R-factor | 0.217 |
Rwork | 0.214 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fiq |
RMSD bond length | 0.015 |
RMSD bond angle | 1.659 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 141.420 | 2.668 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 119504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 7.2 | 298 | PEG 8000, pH 7.2, batch, temperature 298K |