3EU9

The ankyrin repeat domain of Huntingtin interacting protein 14

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796, 0.9794
Spacegroup name	P 61 2 2
Unit cell lengths	106.660, 106.660, 255.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.270 - 1.990
R-factor	0.222
Rwork	0.222
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.050
High resolution limit [Å]	1.990	1.990
Rmerge	0.078	0.404
Number of reflections	59687
<I/σ(I)>	14
Completeness [%]	99.1	99.9
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	289	100 mM (NH4)2SO4, 25% polyethylene glycol (PEG) 3350, 0.1M Bis-Tris, pH 5.5. , VAPOR DIFFUSION, HANGING DROP, temperature 289K
1		5.5	289	100 mM (NH4)2SO4, 25% polyethylene glycol (PEG) 3350, 0.1M Bis-Tris, pH 5.5. , VAPOR DIFFUSION, HANGING DROP, temperature 289K