3EU4
Crystal Structure of BdbD from Bacillus subtilis (oxidised)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.863, 43.113, 54.718 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 52.200 - 2.300 |
| R-factor | 0.19601 |
| Rwork | 0.193 |
| R-free | 0.24821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eu3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.262 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.200 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 7198 | |
| <I/σ(I)> | 15.7 | 7.3 |
| Completeness [%] | 94.8 | 81.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG2000, 0.1M ammonium acetate, 0.1M MES, pH6.5, cryo-protected with 20% Ethylene glycol |
