3EU3
Crystal Structure of BdbD from Bacillus subtilis (reduced)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.774, 43.399, 54.658 |
| Unit cell angles | 90.00, 107.40, 90.00 |
Refinement procedure
| Resolution | 26.080 - 1.500 |
| R-factor | 0.18776 |
| Rwork | 0.186 |
| R-free | 0.21919 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BP3 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.210 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 27450 | |
| <I/σ(I)> | 15 | 3.4 |
| Completeness [%] | 97.8 | 89.5 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 27% PEG2000, 0.1M ammonium acetate, 0.1M MES, pH6.5, 1mM DTT,cryo-protected with 20% Ethylene glycol |
