3EU1
Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.678, 66.896, 157.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.080 - 3.000 |
R-factor | 0.21906 |
Rwork | 0.213 |
R-free | 0.27796 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d1a |
RMSD bond length | 0.022 |
RMSD bond angle | 2.095 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 9607 | |
<I/σ(I)> | 2.9 | 0.7 |
Completeness [%] | 89.7 | 93 |
Redundancy | 4.12 | 3.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2micro litres of Hb with 2micro litre of well solution containing 25mM of Phosphate buffer at pH 7.5 with 0.5M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |