Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 61 |
| Unit cell lengths | 59.537, 59.537, 186.092 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.800 - 2.000 |
| R-factor | 0.237 |
| Rwork | 0.221 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3etw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.340 |
| Number of reflections | 25002 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | 0.1M Sodium citrate pH 5.2, and 0.1M Trimethylamine HCl, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
