3ETU
Crystal structure of yeast Dsl1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 110.620, 110.620, 77.203 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.23082 |
| Rwork | 0.229 |
| R-free | 0.26607 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 21447 | |
| <I/σ(I)> | 28.7 | 2.8 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M HEPES, pH 7.5, 0.45-0.50 M sodium citrate, VAPOR DIFFUSION, temperature 298.0K |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M HEPES, pH 7.5, 0.45-0.50 M sodium citrate, VAPOR DIFFUSION, temperature 298.0K |
