3ETL
RadA recombinase from Methanococcus maripaludis in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.100, 81.100, 108.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.220 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 15276 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 96.8 | 72 |
| Redundancy | 9.9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 294 | 6-8% PEG 3350, 0.1 M NH4Cl, 0.05 M MgCl2, 0.05 M Tris-HCl, 1 mM AMPPNP, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
