3ETH
Crystal structure of E. coli Purk in complex with MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.101, 57.109, 59.215 |
| Unit cell angles | 77.46, 82.39, 77.26 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b6s |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.098 |
| Number of reflections | 87837 | |
| <I/σ(I)> | 29.5 | 6.4 |
| Completeness [%] | 93.8 | 81.9 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20-25% monomethylether poly(ethylene glycol) 5000, 10 mM MgATP, 10 mM aminopyrazole ribonucleotide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
