3ETE
Crystal structure of bovine glutamate dehydrogenase complexed with hexachlorophene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.374, 101.577, 166.873 |
| Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.062 | 0.030 | 0.397 |
| Number of reflections | 156833 | ||
| <I/σ(I)> | 20.143 | ||
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M sodium phosphate (pH 7.0), 0.5 M 1,6-hexanediol, 0.5 M sodium chloride, and 7-8% (w/v) polyethylene glycol 8000., hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
