3ETE
Crystal structure of bovine glutamate dehydrogenase complexed with hexachlorophene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.374, 101.577, 166.873 |
Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.26600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.062 | 0.030 | 0.397 |
Number of reflections | 156833 | ||
<I/σ(I)> | 20.143 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M sodium phosphate (pH 7.0), 0.5 M 1,6-hexanediol, 0.5 M sodium chloride, and 7-8% (w/v) polyethylene glycol 8000., hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |