3ESW
Complex of yeast PNGase with GlcNAc2-IAc.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 131.530, 131.530, 127.751 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.960 - 3.400 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23500 |
Starting model (for MR) | 1x3z |
RMSD bond length | 0.015 |
RMSD bond angle | 1.599 |
Data reduction software | DENZO |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.969 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.126 | 0.457 |
Total number of observations | 20859 | |
Number of reflections | 17986 | |
<I/σ(I)> | 5.496 | 4.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 291 | 0.1 M Tris-HCl, pH 8.5 and 2.0 M sodium chloride, EVAPORATION, temperature 291K |