3ES8
Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 |
Unit cell lengths | 105.584, 105.695, 105.718 |
Unit cell angles | 109.31, 109.47, 109.66 |
Refinement procedure
Resolution | 24.940 - 2.200 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.079 |
Number of reflections | 170773 |
Completeness [%] | 95.7 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 0.15 M DL-malic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |