3ES2
Structure of the C-terminal phosphatase domain of P. aeruginonsa RssB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .97930 |
Spacegroup name | P 31 |
Unit cell lengths | 70.211, 70.211, 92.924 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 13.950 - 2.950 |
R-factor | 0.259 |
Rwork | 0.257 |
R-free | 0.29600 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.756 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 13.950 |
High resolution limit [Å] | 2.950 |
Number of reflections | 19831 |
Completeness [%] | 92.6 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.0 M Lithium Sulfate, 0.1 M MES, cryo-protected with 25% glycerol in well solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.0 M Lithium Sulfate, 0.1 M MES, cryo-protected with 25% glycerol in well solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |