3ERB
The Crystal Structure of C2b, a Fragment of Complement Component C2 produced during C3-convertase Formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.060, 60.060, 61.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.520 - 1.800 |
| R-factor | 0.21552 |
| Rwork | 0.214 |
| R-free | 0.23963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ok5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.243 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.237 |
| Number of reflections | 23095 | |
| <I/σ(I)> | 21 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.36 | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 3350, 0.22M Sodium Chloride, 0.02M Betaine Hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
