3ER7

Crystal structure of NTF2-like protein of unknown function (YP_001812677.1) from EXIGUOBACTERIUM SP. 255-15 at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97920
Spacegroup name	P 21 21 2
Unit cell lengths	78.680, 91.680, 33.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.514 - 1.500
R-factor	0.178
Rwork	0.176
R-free	0.20900
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.514		1.610
High resolution limit [Å]	1.500	3.330	1.550
Rmerge	0.039	0.019	0.425
Number of reflections	40169	6859	7230
<I/σ(I)>	12.44	34.9	1.9
Completeness [%]	98.8	98.2	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2000M NaCl, 30.0000% PEG-3000, 0.1M TRIS pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K