3ER6
Crystal structure of a putative transcriptional regulator protein from Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97958 |
Spacegroup name | P 31 |
Unit cell lengths | 105.268, 105.268, 101.745 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.625 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.287 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.110 | 0.589 |
Total number of observations | 87086 | |
Number of reflections | 99393 | |
<I/σ(I)> | 8.7 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 2.1M DL-malic acid pH 7, pH 7.0, Vapor diffusion, temperature 294K |