3ER0
Crystal structure of the full length eIF5A from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 59.664, 59.664, 339.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.450 - 3.350 |
| R-factor | 0.3916 |
| Rwork | 0.386 |
| R-free | 0.44824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x6o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.149 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 3.530 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.082 | 0.460 |
| Number of reflections | 5431 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 92.1 | 89.5 |
| Redundancy | 5.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M MES, 30% PEG400, 0.1M MgCl2, 3% Glicerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
