3EQZ
Crystal structure of a response regulator from Colwellia psychrerythraea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97958 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 55.573, 55.573, 173.313 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.25000 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.602 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.374 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.099 | 0.355 |
Total number of observations | 38073 | |
Number of reflections | 17720 | |
<I/σ(I)> | 17.1 | 6.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.9 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 294 | 100mM Hepes pH 8.0, 900mM tri-sodium citrate, Vapor diffusion, temperature 294K |