3EQO
Crystal structure of beta-1,3-glucanase from Phanerochaete chrysosporium (Lam55A) gluconolactone complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.534, 67.146, 109.778 |
| Unit cell angles | 93.94, 106.76, 97.08 |
Refinement procedure
| Resolution | 31.500 - 2.250 |
| R-factor | 0.14473 |
| Rwork | 0.142 |
| R-free | 0.20019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 77511 | |
| <I/σ(I)> | 10.9 | 2.7 |
| Completeness [%] | 91.9 | 74.5 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 15% PEG 3350, 0.1M zinc acetate, 0.05M MES-NaOH, 10% glycerol, 2% ethanol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
