3EQE
Crystal structure of the YubC protein from Bacillus subtilis. Northeast Structural Genomics Consortium target SR112.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 65.830, 65.830, 197.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.510 - 2.820 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.086 | 0.633 |
| Number of reflections | 19930 | |
| <I/σ(I)> | 22.89 | 3.58 |
| Completeness [%] | 98.9 | 92.3 |
| Redundancy | 14.24 | 9.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 18% PEG 4000, 0.1M KAcetate, 0.05M MES, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
