3EQA
Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.826, 73.222, 106.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18374 |
| Rwork | 0.181 |
| R-free | 0.22913 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gly |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.292 |
| Number of reflections | 35298 | |
| <I/σ(I)> | 21.9 | 2 |
| Completeness [%] | 96.5 | 71.6 |
| Redundancy | 4.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 50 mM Tris-acetate (pH 8.5), 22.5 % PEG 6000, 0.4 M sodium acetate, 10 % glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
