3EP4
Human AdoMetDC E256Q mutant with no putrescine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.706, 55.897, 74.329 |
| Unit cell angles | 90.00, 109.84, 90.00 |
Refinement procedure
| Resolution | 42.710 - 1.890 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.890 | 1.880 |
| Rmerge | 0.049 | 0.396 |
| Number of reflections | 26047 | |
| <I/σ(I)> | 15.966 | 2 |
| Completeness [%] | 96.7 | 87 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 16% PEG 8000, 100 mM Tris, 10 mM DTT, pH 8.0, vapor diffusion, hanging drop, temperature 295K |
