3EP1
Structure of the PGRP-Hd from Alvinella pompejana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.360, 71.850, 141.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.960 - 2.100 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yck |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.260 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 21905 | |
<I/σ(I)> | 15.7 | 5.7 |
Completeness [%] | 98.5 | 96.7 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 200mM Sodium Tartrate, 20% PEG3350, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |