3EOY
Structure of Reovirus sigma1 in Complex with Its Receptor Junctional Adhesion Molecule-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-26 |
Detector | MAR225 CCD |
Wavelength(s) | 0.91988 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.940, 124.340, 130.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.400 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2OJ5 and 1NBQ |
RMSD bond length | 0.011 |
RMSD bond angle | 1.543 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.163 | 0.212 |
Number of reflections | 21974 | |
<I/σ(I)> | 6.933 | 2.7 |
Completeness [%] | 90.2 | 69.7 |
Redundancy | 3.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES, 30% PEG 3350, streak seeding, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |