3EOP
Crystal Structure of the DUF55 domain of human thymocyte nuclear protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 51.258, 51.258, 122.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.390 - 2.300 |
| Rwork | 0.182 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ar1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.410 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.291 |
| Number of reflections | 15931 | |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.9 | 291 | 0.1M NaAc (pH 4.0-4.8), 28% PEG 2000 MME, 200mM (NH4)2SO4, 3% 1,6-diaminohexane as additive, pH 10.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
