3EOJ
Fmo protein from Prosthecochloris Aestuarii 2K AT 1.3A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 63 |
Unit cell lengths | 111.242, 111.242, 98.198 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.300 |
R-factor | 0.1365 |
Rwork | 0.135 |
R-free | 0.16130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1BCL |
RMSD bond length | 0.013 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.074 | 0.596 |
Number of reflections | 168292 | |
<I/σ(I)> | 26.5 | 2.77 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Well Solution: 10mM Tris/HCL pH 8.0, 1.0M NaCl, 10% w/v (NH4)2SO4. Protein Solution: 10mM Tris/HCL pH 7.8 1.0M NaCl at 14mg/ml of complex. Drop composed of 5ul of protein solution and 3ul of well solution., VAPOR DIFFUSION, HANGING DROP, temperature 298K |