3EOI
CRYSTAL STRUCTURE OF putative PROTEIN PilM from Escherichia coli B7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.754, 54.213, 50.091 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.520 |
| R-factor | 0.19203 |
| Rwork | 0.190 |
| R-free | 0.23688 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.689 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.600 |
| High resolution limit [Å] | 1.520 | 4.260 | 1.570 |
| Rmerge | 0.104 | 0.071 | 0.409 |
| Number of reflections | 34498 | ||
| <I/σ(I)> | 11.628 | ||
| Completeness [%] | 98.0 | 99.4 | 87.7 |
| Redundancy | 3.9 | 4.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1M bis-tris, ph 5.5, 2.0M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
