3EOH
Refined group II intron structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-17 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.092, 94.969, 225.972 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.705 - 3.125 |
R-factor | 0.2095 |
Rwork | 0.208 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.397 |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.705 |
High resolution limit [Å] | 3.125 |
Number of reflections | 34739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 303 | 6% PEG 3350, 150 mM magnesium acetate, 350 mM KCL, 50 mM Na-HEPES pH 7.0, 0.5 mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | KCL | ||
4 | 1 | 1 | Na-HEPES | ||
5 | 1 | 1 | spermine | ||
6 | 1 | 2 | PEG 335 | ||
7 | 1 | 2 | magnesium acetate | ||
8 | 1 | 2 | KCL |