3EOG
Co-crystallization showing exon recognition by a group II intron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.485, 95.078, 225.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.419 - 3.391 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.488 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.600 |
| High resolution limit [Å] | 3.391 |
| Number of reflections | 27468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 303 | 6% PEG 3350, 150 mM magnesium acetate, 350 mM KCL, 50 mM Na-HEPES pH 7.0, 0.5 mM spermine, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 1 | Na-HEPES | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | magnesium acetate | ||
| 8 | 1 | 2 | KCL |
