3EOE
Crystal Structure of Pyruvate Kinase from toxoplasma gondii, 55.m00007
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.039, 130.684, 113.848 |
Unit cell angles | 90.00, 117.40, 90.00 |
Refinement procedure
Resolution | 42.680 - 2.310 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.069 | 0.588 |
Number of reflections | 103408 | |
<I/σ(I)> | 1.2 | |
Completeness [%] | 93.2 | 53.8 |
Redundancy | 3.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |