3EOD
Crystal structure of N-terminal domain of E. coli RssB
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932,1.0070,1.0087 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 45.114, 45.114, 96.797 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.070 - 1.750 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.839 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.061 | 0.044 | 0.464 |
Number of reflections | 12003 | ||
<I/σ(I)> | 80.733 | ||
Completeness [%] | 99.1 | 94.8 | 98 |
Redundancy | 38.6 | 31.3 | 23.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.75 | 298 | 0.1 M Tris-HCl, 0.2 Na Acetate, 1 mM DTT, 28% PEG3350, 1.9 mg/ml protein, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K |