3EO5
Crystal structure of the resuscitation promoting factor RpfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9464 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 46.053, 105.445, 121.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.043 | 0.232 |
| Number of reflections | 27100 | |
| <I/σ(I)> | 37.9 | 3.3 |
| Completeness [%] | 94.5 | 70.1 |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | pH 6.5, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | pH 6.5, VAPOR DIFFUSION, temperature 293K |
