3ENI
Crystal structure of the Fenna-Matthews-Olson Protein from Chlorobaculum Tepidum
Replaces: 1M50Replaces: 1KSAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 169.100, 169.100, 169.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.195 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.129 | 0.742 |
| Number of reflections | 41641 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 87.0 | 63.2 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 6% (W/V) POLYETHYLENE GLYCOL 600, 50 MM SODIUM CITRATE, 10% (V/V) 2-PROPONAL, pH 5.60, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
