3ENH

Crystal structure of Cgi121/Bud32/Kae1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-23
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	76.990, 106.910, 209.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.920 - 3.600
R-factor	0.27371
Rwork	0.271
R-free	0.32410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ARCHEAL CGI121 AND ARCHEAL KAE1/BUD32 STRUCTURES
RMSD bond length	0.047
RMSD bond angle	1.369
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	208.840	3.700
High resolution limit [Å]	3.600	3.600
Rmerge	0.075	0.217
Number of reflections	20565
<I/σ(I)>	11.96	3.4
Completeness [%]	99.8
Redundancy	1.86	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES and 10-15% PEG 3000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K