3ENC
Crystal structure of Pyrococcus furiosus PCC1 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 63 |
| Unit cell lengths | 78.810, 78.810, 60.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.251 - 2.630 |
| R-factor | 0.1921 |
| Rwork | 0.192 |
| R-free | 0.22210 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.232 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.251 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.033 | 0.268 |
| Number of reflections | 6401 | |
| <I/σ(I)> | 20.16 | 2.86 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 1.93 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 45% PEG 300 0.1 M Na/K phosphate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
