3EN3
Crystal Structure of the GluR4 Ligand-Binding domain in complex with kainate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.906, 48.749, 47.766 |
Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
Resolution | 11.950 - 2.430 |
R-factor | 0.159 |
Rwork | 0.156 |
R-free | 0.21900 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.173 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.950 | 2.490 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 10025 | |
Completeness [%] | 95.3 | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 25.5% PEG 1500, 0.05M Na-Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |