3EMU
Crystal structure of a leucine rich repeat and phosphatase domain containing protein from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.985, 80.985, 90.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.553 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.042 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.077 | 0.453 |
| Total number of observations | 22302 | |
| Number of reflections | 15704 | |
| <I/σ(I)> | 15.2 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 500mM ammonium sulfate, 1M lithium sulfate, 100mM tri-sodium citrate dihydrate, pH 7.0, Vapor diffusion, temperature 294K, VAPOR DIFFUSION |
