3EMS
Effect of Ariginine on lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-15 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.357, 78.357, 37.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.690 - 1.651 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hel |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.979 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.690 | 33.690 | 1.740 |
| High resolution limit [Å] | 1.651 | 5.220 | 1.650 |
| Rmerge | 0.048 | 0.033 | 0.107 |
| Total number of observations | 4325 | 1983 | |
| Number of reflections | 13095 | ||
| <I/σ(I)> | 11.182 | 15.4 | 6.5 |
| Completeness [%] | 90.3 | 99.7 | 48.7 |
| Redundancy | 6.4 | 8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 1.5M NaCl, 0.5M Arg, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
