3EMS

Effect of Ariginine on lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-07-15
Detector	RIGAKU RAXIS
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.357, 78.357, 37.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.690 - 1.651
R-factor	0.207
Rwork	0.206
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hel
RMSD bond length	0.006
RMSD bond angle	0.979
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.690	33.690	1.740
High resolution limit [Å]	1.651	5.220	1.650
Rmerge	0.048	0.033	0.107
Total number of observations		4325	1983
Number of reflections	13095
<I/σ(I)>	11.182	15.4	6.5
Completeness [%]	90.3	99.7	48.7
Redundancy	6.4	8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	1.5M NaCl, 0.5M Arg, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K