3EMO
Crystal structure of transmembrane Hia 973-1098
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-01-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.300, 45.811, 56.423 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 29.570 - 3.000 |
| Rwork | 0.221 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gr7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.984 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | 0.150 |
| Number of reflections | 8519 | |
| <I/σ(I)> | 11.1 | 4.1 |
| Completeness [%] | 84.6 | 60.8 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 16% PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
