3EMC
Crystal structure of XynB, an intracellular xylanase from Paenibacillus barcinonensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-10-25 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.930, 79.110, 91.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.100 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hiz |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.115 | 0.469 |
| Number of reflections | 20647 | |
| <I/σ(I)> | 9.922 | |
| Completeness [%] | 97.7 | 80 |
| Redundancy | 7.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 35% (v/v) MPD, 17% (w/v) PEG 3350, 100mM tris, VAPOR DIFFUSION, temperature 298K |
