3EM0
Crystal structure of Zebrafish Ileal Bile Acid-Bindin Protein complexed with cholic acid (crystal form B).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.285, 85.285, 75.055 |
| Unit cell angles | 90.00, 127.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.2272 |
| Rwork | 0.226 |
| R-free | 0.25422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3elz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.536 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.312 |
| Number of reflections | 24125 | |
| <I/σ(I)> | 9.1 | 1.3 |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Sodium HEPES, 2.0 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
