3ELV

Crystal Structure of Full-Length Yeast Pml1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9802
Spacegroup name	P 32 2 1
Unit cell lengths	86.891, 86.891, 96.697
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.22027
Rwork	0.218
R-free	0.26592
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	16896
<I/σ(I)>	19.7	1.6
Completeness [%]	99.9	100
Redundancy	6.3	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM Tris-HCl, pH 7.5, 200 mM Li2SO4, 21-24 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K