3ELI
Crystal structure of the AHSA1 (SPO3351) protein from Silicibacter pomeroyi, Northeast Structural Genomics Consortium Target SiR160
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97845 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.348, 73.348, 91.891 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.800 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26500 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.147 | 0.581 |
Number of reflections | 11776 | |
<I/σ(I)> | 21.7 | 2.2 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 13.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100 mM TAPS (pH 9) and 8.64 M potassium acetate. VAPOR DIFFUSION, HANGING DROP. |