3EKS

Crystal Structure of Monomeric Actin bound to Cytochalasin D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-01-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	195.207, 54.634, 38.569
Unit cell angles	90.00, 90.65, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.25
Rwork	0.237
R-free	0.28400
RMSD bond length	0.007
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.125	0.363
Number of reflections	31036
<I/σ(I)>	12.6	1.89
Completeness [%]	81.3	54.8
Redundancy	3.1	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, MICROSEEDING, MACROSEEDING	5.2	277	15.5% MPD, 0.1M sodium acetate, 0.1M NaCl, 0.02M CaCl2, pH 5.2, VAPOR DIFFUSION, SITTING DROP, MICROSEEDING, MACROSEEDING, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP, MICROSEEDING, MACROSEEDING	5.2	277	15.5% MPD, 0.1M sodium acetate, 0.1M NaCl, 0.02M CaCl2, pH 5.2, VAPOR DIFFUSION, SITTING DROP, MICROSEEDING, MACROSEEDING, temperature 277K