3EKI

Structural insights of the Mycoplasma hyorhinis protein Mh-p37: A putative thiamine pyrophosphate transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9169
Spacegroup name	P 1 21 1
Unit cell lengths	49.457, 67.586, 59.838
Unit cell angles	90.00, 106.28, 90.00

Refinement procedure

Resolution	40.000 - 1.600
Rwork	0.183
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e78
RMSD bond length	0.008
RMSD bond angle	0.024
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	47500
<I/σ(I)>	0.115	3.1
Completeness [%]	94.6	95.6
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	4	298	0.1 M Ammonium Bromide, 0.1 M Citric Acid, 40% PEG 4000, under paraffin oil, pH 4.0, Batch, temperature 298K