3EKI
Structural insights of the Mycoplasma hyorhinis protein Mh-p37: A putative thiamine pyrophosphate transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9169 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.457, 67.586, 59.838 |
| Unit cell angles | 90.00, 106.28, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| Rwork | 0.183 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e78 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 47500 | |
| <I/σ(I)> | 0.115 | 3.1 |
| Completeness [%] | 94.6 | 95.6 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 4 | 298 | 0.1 M Ammonium Bromide, 0.1 M Citric Acid, 40% PEG 4000, under paraffin oil, pH 4.0, Batch, temperature 298K |
