3EKF
Crystal structure of the A264Q heme domain of cytochrome P450 BM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.067, 119.709, 146.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.100 |
| R-factor | 0.21786 |
| Rwork | 0.215 |
| R-free | 0.26682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ij4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.358 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.088 |
| Number of reflections | 59671 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Magnesium sulphate and PEG, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
