3EK9
SPRY Domain-containing SOCS Box Protein 2: Crystal Structure and Residues Critical for Protein Binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.255, 68.176, 69.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.690 - 2.600 |
| R-factor | 0.17277 |
| Rwork | 0.168 |
| R-free | 0.27269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fnj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.117 | 0.356 |
| Number of reflections | 5002 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 94.0 | 93.9 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 100mM Bis-Tris, pH6.5, 200mM MgCl2, 25% PEG3350, EVAPORATION, temperature 293K |
