3EK8
Calcium-saturated GCaMP2 T116V/G87R mutant monomer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-12 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 120.800, 120.800, 97.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.300 - 2.800 |
R-factor | 0.22569 |
Rwork | 0.224 |
R-free | 0.26553 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ek7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.355 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.300 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 18310 | |
<I/σ(I)> | 3.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.94 | 12.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1 M Magnesium formate dihydrate, 15% w/v Polyethylene glycol 3,350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |